Primer-BLAST: A tool to design target-specific primers for …
Primer-Blast achieves this result by using highly sensitive BLAST parameters as well as an additional NW global alignment algorithm to ensure a complete alignment between the primer …
Electronic-PCR (e-PCR) is retiring, use Primer-BLAST instead
Jun 28, 2017 · NCBI is retiring the e-PCR tool effective immediately. The good news is that an existing tool, Primer-BLAST, fills in nicely for the functions of both Forward and Reverse e …
Primer-BLAST: a tool to design target-specific primers for
Jun 18, 2012 · We describe a robust and fully implemented general purpose primer design tool that designs target-specific PCR primers. Primer-BLAST offers flexible options to adjust the …
Basic Local Alignment Search Tool - BLAST
Jul 21, 2025 · The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional …
Using NCBI’s Primer-BLAST to design and analyze PCR primers
Sep 26, 2023 · Primer-BLAST primers are suitable for use in all PCR based molecular biology protocols including target identification / verification, cloning, variant analysis, and gene …
Primer-Blast results - National Center for Biotechnology Information
Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target.
Finding primers specific to your PCR template (using Primer3 and …
Enter the PCR template here (multiple templates are currently not supported). It is highly recommended to use refseq accession or GI (rather than the raw DNA sequence) whenever …
Real-Time Quantitative PCR: Primer Design, Reference Gene …
Real-time quantitative PCR, also called quantitative PCR (qPCR), combines PCR and fluorescent dyes to monitor template amplification with high sensitivity and specificity.
Primer Design for Reverse Transcription-Quantitative PCR …
Here, we describe a relatively simple online lab for students in introductory biology classes that can be completed in one session using NCBI Primer-BLAST and a virtual PCR tool …
Using NCBI’s Primer-BLAST to design and analyze PCR primers
Jun 14, 2022 · NCBI's Primer-BLAST combines the primer design features of the popular Primer3 package with a specificity check that uses nucleotide BLAST to search for target and non …