Jul 21, 2025 · The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and …

Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target.

For each gene, at least one primer pair has been designed and in many cases alternative primer pairs exist. Primers have been designed to work under the same PCR conditions, thus facilitating high …

Primer choice influences the estimated microbial composition. A set of different 16S rRNA gene sequencing primer pairs covering one, two, or three of the variable regions V1 to V9 is commonly …

Here, we describe a novel empirical-based method, miPrimer, which greatly reduces primer dimerization and increases primer specificity by factoring various intrinsic primer properties and employing four …

Users can design new primer pairs by entering the DNA template alone or they can design one primer by entering the template plus the other pre-existing primer. Primer-BLAST can check the specificity …

The option "Primer must span an exon-exon junction" will direct the program to return at least one primer (within a given primer pair) that spans an exon-exon junction.

Jun 18, 2012 · We describe a robust and fully implemented general purpose primer design tool that designs target-specific PCR primers. Primer-BLAST offers flexible options to adjust the specificity …

In this primer, we discuss the ways in which language models, both those based on natural language and those based on biological sequences, can be applied to biological research.

Jun 28, 2017 · The good news is that an existing tool, Primer-BLAST, fills in nicely for the functions of both Forward and Reverse e-PCR, and has the additional benefit of de novo primer design.